Shimadzu BL3200S Bedienerhandbuch

228-90747Aug. 2009Read the instruction manual thoroughly before you use the product.Keep this instruction manual for future reference.SPECTROFLUOROMET

IntroductionVIIIRF-20A/20Axs Operation Precautions!WARNING• Take thorough measures to prevent buildup of static electricity.^ "Static Electricit

4. Basic Operation4-42RF-20A/20AxsThis page is intentionally left blank.

55 Application OperationContents5.1 Types of Screen and Their Explanations ... 5-25.2 Setting t

5. Application Operation5-2RF-20A/20Axs5.1 Types of Screen and Their Explanations5.1.1 Types of ScreenOn turning the power ON, the initial screen app

5.1 Types of Screen and Their Explanations5-3RF-20A/20Axs55.1.2 Auxiliary Function ScreenIn this section the auxiliary function screens are shown in

5. Application Operation5-4RF-20A/20AxsAnalog output connector 1 output range setting^ P.5-22ANA1 REC RANGE 1Input0-9Analog output connector 1 baseli

5.1 Types of Screen and Their Explanations5-5RF-20A/20Axs5CONTROLEnter to SelectSpectrum scan file specification ^ P.5-26SCAN FILE 00:BG 1:

5. Application Operation5-6RF-20A/20AxsSpectrum output ^ P. 5- 28SPC PLOTEnter to PlotLocal mode setting ^ P.5-29SYSTEMEnter to SelectLOCAL

5.1 Types of Screen and Their Explanations5-7RF-20A/20Axs5Xenon lamp cumulative operating time indication^ P.5-32Xe TIME 0.0 Xenon lamp ignition cou

5. Application Operation5-8RF-20A/20Axs5.1.3 VP Function ScreenIn this section the VP function screens are shown in the following flow diagrams.There

5.1 Types of Screen and Their Explanations5-9RF-20A/20Axs5DATE YY-MM-DD03-05-12OPERATION LOGEnter to DisplayVALIDATIONPress func or VPLEAK SE

Safety InstructionsIXRF-20A/20Axs Precautions for Instrument Inspection, Maintenance, Adjustment and Care!WARNING• Unplug the instrument before inspe

5. Application Operation5-10RF-20A/20AxsLEAK THR 150ActLv 100/150Leak sensor actuation level entry ^ P.5-45Leak sensor calibration ^ P.5-45L-CA

5.1 Types of Screen and Their Explanations5-11RF-20A/20Axs5Password change ^ P.5-47CBM parameter setting ^ P.5-48∗ Only when connected by link to CB

5. Application Operation5-12RF-20A/20Axs5.2 Setting the Auxiliary FunctionsThe auxiliary functions are functions for setting the parameters for measu

5.2 Setting the Auxiliary Functions5-13RF-20A/20Axs55.2.1 List of Auxiliary FunctionsThe auxiliary functions are listed in the tables below.^ "

5. Application Operation5-14RF-20A/20Axs System Settings Group Monitor Display GroupName Operation Function PageLOCAL Numeric keysTo set whether th

5.2 Setting the Auxiliary Functions5-15RF-20A/20Axs55.2.2 Showing the Auxiliary Function Screen1Press .The initial screen will be displayed.2Press

5. Application Operation5-16RF-20A/20Axs5.2.3 Parameter Settings GroupThis is the group that relates to parameter settings. Setting the Wavelength f

5.2 Setting the Auxiliary Functions5-17RF-20A/20Axs5 Setting the Measurement Mode [λ MODE]Set the measurement mode as single wavelength mode or dua

5. Application Operation5-18RF-20A/20Axs Setting the Response [RESPONSE]Set the response.^ "4.1.6 Setting the Response (Response Speed)" P

5.2 Setting the Auxiliary Functions5-19RF-20A/20Axs5 Setting the Sensitivity [SENS]Set the sensitivity of the instrument.^ "4.1.8 Setting Sens

IntroductionXRF-20A/20AxsStatic Electricity PrecautionsLiquid chromatograph (LC) uses flammable organic solvent(s) as the mobile phase. LC systems are

5. Application Operation5-20RF-20A/20Axs Setting the Output Mode for Analog Output Connector 1 [ANALOG1 MODE]RF-20ASet whether a Chromatopac or a re

5.2 Setting the Auxiliary Functions5-21RF-20A/20Axs5 Setting the Output Mode for Analog Output Connector 2 [ANALOG2 MODE]RF-20ASet whether a Chroma

5. Application Operation5-22RF-20A/20Axs Setting the Output Range for Analog Output Connector 1 [ANA1 REC RANGE]Set the output range for analog outp

5.2 Setting the Auxiliary Functions5-23RF-20A/20Axs5 Setting the Output Range for Analog Output Connector 2 [ANA2 REC RANGE]Set the output range fo

5. Application Operation5-24RF-20A/20Axs Setting the Baseline Offset Value for Analog Output Connector 1 [BL OFS ANA1]Set the baseline offset value

5.2 Setting the Auxiliary Functions5-25RF-20A/20Axs5 Setting the EVENT Output Terminal [EVENT]Set the operation of the EVENT output terminal.^ &quo

5. Application Operation5-26RF-20A/20Axs5.2.4 Control Settings GroupThis is the group that relates to control of this instrument. Setting the File N

5.2 Setting the Auxiliary Functions5-27RF-20A/20Axs5 Setting the Start and End Emission Wavelengths [EM SCAN]Set the start and end emission wavelen

5. Application Operation5-28RF-20A/20Axs Setting the Scan Speed [SCAN SPEED]Set the scan speed for spectrum scanning.Enter the set value from the nu

5.2 Setting the Auxiliary Functions5-29RF-20A/20Axs55.2.5 System Settings GroupThis is the group that relates to system settings, such as connection

Static Electricity PrecautionsXIRF-20A/20Axs Preventing Static Electricity AccidentsThe best way to prevent static electricity accidents is simply to

5. Application Operation5-30RF-20A/20Axs Adjusting the Brightness of the Display Screen [BRIGHTNESS]Adjust the contrast of the display screen in fou

5.2 Setting the Auxiliary Functions5-31RF-20A/20Axs5 Displaying the Program Elapsed Time [MONIT-TIME]Set whether to display the time elapsed since

5. Application Operation5-32RF-20A/20AxsUpon entering the cell number set for this instrument, the instrument operates based on the wavelength calibr

5.2 Setting the Auxiliary Functions5-33RF-20A/20Axs5 Displaying the Xenon Lamp Ignition Count [Xe COUNT]Displays the ignition count of the Xenon la

5. Application Operation5-34RF-20A/20Axs5.3 Setting the VP FunctionsThe VP functions support the validation of the instrument through the display of

5.3 Setting the VP Functions5-35RF-20A/20Axs5 Validation Support Group Calibration Support Group* For the VP functions in the calibration support

5. Application Operation5-36RF-20A/20Axs5.3.2 Showing the VP Function Screen1Press .The initial screen will be displayed.2Press to select the desir

5.3 Setting the VP Functions5-37RF-20A/20Axs55.3.3 Product Information GroupThis is the group that relates to information. Showing the Serial Numbe

5. Application Operation5-38RF-20A/20Axs Showing the Replacement Alert Time for the Xenon Lamp, and Its Cumulative Operating Time [Xe LAMP USED TM]S

5.3 Setting the VP Functions5-39RF-20A/20Axs5 Showing the Maintenance Log [MAINTENANCE LOG]Shows the last 20 records, including the part numbers of

IntroductionXIIRF-20A/20AxsPreventive Measure 2Cover the spaces between the tubing and the sides of the inlet and outlet openings of the waste contain

5. Application Operation5-40RF-20A/20Axs Showing the Error Log [ERROR LOG]Shows the details of the last 10 errors and their dates of occurrence. Pre

5.3 Setting the VP Functions5-41RF-20A/20Axs55.3.5 Validation Support GroupThis is the group for checking whether this instrument is operating corre

5. Application Operation5-42RF-20A/20AxsExample: Entering 5:30:55 pm1Enter the time in the order hour, minute, second, from the numeric keys. The ent

5.3 Setting the VP Functions5-43RF-20A/20Axs55.3.6 Calibration Support GroupThis is the group for calibrating this instrument.NOTEThe instrument is

5. Application Operation5-44RF-20A/20Axs Executing Wavelength Calibration [WAVE CALIB]Performs wavelength calibration and a wavelength accuracy chec

5.3 Setting the VP Functions5-45RF-20A/20Axs5 Calibrating the Leak Sensor [L-CAL]Calibrate the leak sensor.While the leak sensor is dry, check that

5. Application Operation5-46RF-20A/20Axs Selecting the Operation Mode [OP MODE]Set the operation mode of the instrument according to the system cont

5.3 Setting the VP Functions5-47RF-20A/20Axs5 Changing the Password [CHANGE PASSWORD]Change the password.1Press .The password entry screen will be

5. Application Operation5-48RF-20A/20Axs Showing/Setting CBM Parameters [CBM PARAMETER]This item is shown when the instrument is connected to a CBM-

5.3 Setting the VP Functions5-49RF-20A/20Axs55.3.7 Showing and Setting CBM Parameters (Calibration Support Group)This is the procedure for showing a

Static Electricity PrecautionsXIIIRF-20A/20AxsFor ReferenceAnti-static equipment (anti-static clothing, shoes and matting) and charge measurement equi

5. Application Operation5-50RF-20A/20Axs Showing the Serial Number [SERIAL NUMBER]Shows the serial number of the CBM that controls the instrument.Wi

5.3 Setting the VP Functions5-51RF-20A/20Axs5 Setting the Ethernet Communications Speed [ETHERNET SPEED]Set the communications speed for the Ethern

5. Application Operation5-52RF-20A/20Axs Setting the IP Address [IP ADDRESS]Set the IP address of the CBM that controls this instrument.1With the [C

5.3 Setting the VP Functions5-53RF-20A/20Axs52Enter the set value from the numeric keys and press .NOTEConsult the network administrator about the s

5. Application Operation5-54RF-20A/20Axs5.4 Creating Time ProgramsThis instrument sets parameters such as wavelength, response, output range and so o

5.4 Creating Time Programs5-55RF-20A/20Axs5SCAN Numeric keysTo execute spectrum scanning and save the data in the specified file number.0 to 2 P. 5

5. Application Operation5-56RF-20A/20Axs5.4.2 Time Program Edit ScreenWhen creating a time program, set the necessary parameters on the time program

5.4 Creating Time Programs5-57RF-20A/20Axs5 Example of Creation of a Time ProgramShown here is an example where spectrum scanning is executed 5 min

5. Application Operation5-58RF-20A/20Axs5Press repeatedly until [SCAN] is displayed, then press .6Press and .[SCAN] is executed 5 minutes after t

5.4 Creating Time Programs5-59RF-20A/20Axs55.4.3 Setting the Loop Count of the Program [LOOP]By setting [LOOP], the time program can be repeated the

IntroductionXIVRF-20A/20Axs In an Emergency During a Power Outage!WARNINGIf any problem is detected, such as a burning smell, take the following act

5. Application Operation5-60RF-20A/20Axs5.4.5 Starting a Time ProgramThere are the following two methods for starting a time program.• Press .• Input

5.5 Measuring in the Spectrum Scanning Mode5-61RF-20A/20Axs55.5 Measuring in the Spectrum Scanning ModeThis instrument features a spectrum scanning

5. Application Operation5-62RF-20A/20Axs5.5.2 Overview of Spectrum MeasurementSpectrum measurement is carried out in accordance with the flow shown b

5.5 Measuring in the Spectrum Scanning Mode5-63RF-20A/20Axs55.5.3 Setting the Scan TypeSet whether to use the excitation or emission scanning in spe

5. Application Operation5-64RF-20A/20Axs5.5.4 Setting the Start Wavelength and End WavelengthSet the start wavelength and end wavelength for spectrum

5.5 Measuring in the Spectrum Scanning Mode5-65RF-20A/20Axs55.5.5 Setting the Excitation Wavelength and Emission WavelengthSet the excitation and em

5. Application Operation5-66RF-20A/20Axs5.5.6 Setting the Scan SpeedSet the scan speed for spectrum scanning. Details of the scan speed settings are

5.5 Measuring in the Spectrum Scanning Mode5-67RF-20A/20Axs55.5.7 Setting SensitivityThis is the procedure for setting sensitivity. By combining the

5. Application Operation5-68RF-20A/20Axs5.5.8 Setting GainThis is the procedure for setting gain. By combining the setting of sensitivity and gain, y

5.5 Measuring in the Spectrum Scanning Mode5-69RF-20A/20Axs55.5.9 Setting the Flow Cell Temperature (RF-20Axs Only)Set the temperature of the flow c

Precautions for Mobile Phase Selection and UseXVRF-20A/20AxsPrecautions for Mobile Phase Selection and Use!CAUTION• If PEEK resin parts are used in th

5. Application Operation5-70RF-20A/20Axs5.5.10 Setting the Scan FileThis instrument can save two sample spectra and one background spectrum.First of

5.5 Measuring in the Spectrum Scanning Mode5-71RF-20A/20Axs53Press .[SCAN FILE] will be displayed.4Enter and press .The file number of the file in

5. Application Operation5-72RF-20A/20Axs5.5.11 Measuring Procedure1Press while the flow cell is full of mobile phase.Spectrum scanning will start.D

5.5 Measuring in the Spectrum Scanning Mode5-73RF-20A/20Axs55.5.12 Outputting Spectrum DataThis is the procedure for outputting the read spectrum da

5. Application Operation5-74RF-20A/20Axs To Stop Output Part Way ThroughPress while the screen to the right is displayed.Fig. 5.2Example of output

5.6 Connecting to a CBM-20A or CBM-20Alite System Controller5-75RF-20A/20Axs55.6 Connecting to a CBM-20A or CBM-20Alite System Controller5.6.1 Setti

5. Application Operation5-76RF-20A/20Axs5.7 Connecting to an SCL-10Avp System Controller5.7.1 Setting the InstrumentTo operate this instrument with a

5.7 Connecting to an SCL-10Avp System Controller5-77RF-20A/20Axs55.7.3 Notes on Operation Restrictions in the RF-10AXL Compatibility ModeWhen this

5. Application Operation5-78RF-20A/20Axs Parameter Setting Ranges in the RF-10AXL Compatibility ModeIn the RF-10AXL compatibility mode some function

5.7 Connecting to an SCL-10Avp System Controller5-79RF-20A/20Axs5System settingsLOCAL Numeric keys 0: Remote, 1: Local P. 5 -2 9LINK ADRS Numeric ke

IntroductionXVIRF-20A/20AxsPrecautions for the Xenon Lamp!WARNING• When handling a Xenon lamp, always wear the following protective gear: a protective

5. Application Operation5-80RF-20A/20AxsVP functionGroup Name Operation Setting Range PageProduct information (display only)SERIAL NUMBER Display L∗∗

5.8 Connection to External Input/Output Terminals5-81RF-20A/20Axs55.8 Connection to External Input/Output TerminalsThe "external input/output t

5. Application Operation5-82RF-20A/20Axs5.8.2 Wiring1Strip off 10 mm of insulation from the end of the cable to be connected. Note that this is not n

5.8 Connection to External Input/Output Terminals5-83RF-20A/20Axs5NOTEOne event cable (part No.: 228-28253-91) is provided with this instrument. Whe

5. Application Operation5-84RF-20A/20Axs5.9 Using the Spare Flow Cell Unit / Optional CellWhen this instrument is used with a flow cell unit other th

66 TroubleshootingContents6.1 Troubleshooting and Corrective Action ... 6-26.2 Dealing with

6. Troubleshooting6-2RF-20A/20Axs6.1 Troubleshooting and Corrective ActionThis section describes the probable causes of problems that can arise, and

6.1 Troubleshooting and Corrective Action6-3RF-20A/20Axs6S/N ratio fault (weak signal)This is a flow cell unit fitting fault.• Check whether the flo

6. Troubleshooting6-4RF-20A/20Axs∗1 For details on the method for checking for air bubbles and contamination inside the cell, see:^ "8.2 Inspect

6.2 Dealing with Error Messages6-5RF-20A/20Axs66.2 Dealing with Error MessagesThe instrument has several diagnostic functions. Upon detection of a p

Precautions for the Xenon LampXVIIRF-20A/20Axs!WARNING• Make sure that the Xenon lamp has cooled sufficiently before attempting to replace it.Immediat

6. Troubleshooting6-6RF-20A/20AxsERR EX HOME POSFatalCause: Displayed when the home position sensor of the excitation grating motor is not operating

6.2 Dealing with Error Messages6-7RF-20A/20Axs6ERR HEATERFatalCause: Displayed when the temperature of the flow cell has failed to reach the set te

6. Troubleshooting6-8RF-20A/20AxsNOT PROTECTEDAlarmCause: Displayed if the parameters and time program set for the previous measurement have been lo

6.2 Dealing with Error Messages6-9RF-20A/20Axs6ERR Hg LAMPAlarmCause: Displayed when the low-pressure Hg (mercury) lamp cannot be turned on.(Low-pr

6. Troubleshooting6-10RF-20A/20AxsERR CELL TEMPAlarmCause: Displayed when the flow cell temperature has risen abnormally.(Flow cell temperature erro

6.2 Dealing with Error Messages6-11RF-20A/20Axs6ERR FAN STOPAlarmCause: Displayed when the cooling fan for the Xenon lamp's power supply stops

6. Troubleshooting6-12RF-20A/20AxsNO PEAKS 1WarningCause: Displayed if, on executing [S/N CHECK], no raman spectrum peak was detected at an emission

6.2 Dealing with Error Messages6-13RF-20A/20Axs6ERR FILE TYPEWarningCause: Displayed when the spectrum types or wavelength ranges of the data in fi

6. Troubleshooting6-14RF-20A/20AxsLOW SET TEMPWarningCause: Displayed if the room temperature exceeds the set temperature for the flow cell by 10 °C

77 Hardware ValidationThis chapter explains the inspection procedures for periodically checking that individualcomponents and the instrument as a who

This page is intentionally left blank.

IntroductionXVIIIRF-20A/20AxsPrecautions on Replacing Fuses!WARNING• Before replacing fuses, turn the power to the instrument OFF and unplug the instr

7. Hardware Validation7-2RF-20A/20Axs7.1 Overview of Hardware Validation7.1.1 Hardware ValidationHardware validation is the work of inspecting whethe

7.2 Implementation of Hardware Validation7-3RF-20A/20Axs77.2 Implementation of Hardware Validation7.2.1 Periodic InspectionComponent and system vali

7. Hardware Validation7-4RF-20A/20Axs7.3 Precautions on Validation7.3.1 EnvironmentData reproducibility may be adversely affected by abrupt changes i

7.4 Equipment Required for Validation7-5RF-20A/20Axs77.4 Equipment Required for ValidationThe equipment and reagents listed below are required for h

7. Hardware Validation7-6RF-20A/20Axs Standard Reagents for ValidationA list of standard reagents required for validation is shown below. The custom

7.5 Detector Validation7-7RF-20A/20Axs77.5 Detector Validation7.5.1 Check PointsThe check points for detector validation are listed below.Section Ch

7. Hardware Validation7-8RF-20A/20Axs7.5.2 Initialization Check and Self Diagnosis of the ROM and RAM ObjectiveCheck the operation of the display, L

7.5 Detector Validation7-9RF-20A/20Axs77.5.3 Checking the Firmware Version ObjectiveCheck the version of the firmware. Check Procedure1Press on

7. Hardware Validation7-10RF-20A/20Axs7.5.4 Checking the Cumulative Operating Time of the Xenon Lamp ObjectiveCheck the cumulative operating time of

7.5 Detector Validation7-11RF-20A/20Axs77.5.5 Checking Wavelength Accuracy ObjectiveCheck whether the difference between the set wavelength and the

Disposal PrecautionsXIXRF-20A/20AxsDisposal PrecautionsWhen disposing of the instrument and Xenon lamps, contact your Shimadzu representative. If you

7. Hardware Validation7-12RF-20A/20Axs Check Procedure (for RF-20Axs)NOTETo discontinue the inspection, press .1When a system controller is being u

7.5 Detector Validation7-13RF-20A/20Axs77Press .After waiting about 2 minute for the low-pressure Hg (mercury) lamp to stabilize, [EX CHECK] or [EM

7. Hardware Validation7-14RF-20A/20Axs[EM CHECK][EX CHECK]• If there is an abnormality:The screen to the right appears.^ "6.2 Dealing with Error

7.5 Detector Validation7-15RF-20A/20Axs7When a system controller or LCsolution is used, perform the following operation before analyzing the sample.

7. Hardware Validation7-16RF-20A/20Axs Check Procedure (for RF-20A)NOTETo discontinue the inspection, press .1When a system controller is being use

7.5 Detector Validation7-17RF-20A/20Axs7[EX CHECK]10Repeatedly press until [EX CHECK] or [EM CHECK] appears.[EM CHECK]NOTEFor a wavelength accurac

7. Hardware Validation7-18RF-20A/20Axs• If it is normal:If the difference in wavelength is within the standard, [CHECK GOOD] and the actually measure

7.5 Detector Validation7-19RF-20A/20Axs7[EM CHECK][EX CHECK]• If there is an abnormality:The screen to the right appears.^ "6.2 Dealing with Er

7. Hardware Validation7-20RF-20A/20AxsNOTEAfter the wavelength check has ended, be sure to remove the low-pressure Hg (mercury) lamp, and fit the Xen

7.5 Detector Validation7-21RF-20A/20Axs7NOTEBefore starting analysis when using a system controller or LCsolution, be sure to set [0] for [LOCAL] in

IntroductionXXRF-20A/20AxsWarning LabelsFor safety operation, warning labels are affixed to where special attention is required.Should any of these la

7. Hardware Validation7-22RF-20A/20Axs5Remove the flow cell unit from the instrument.Check that there are no air bubbles in the cell.6Fit the flow ce

7.5 Detector Validation7-23RF-20A/20Axs7 Check Procedure (When Using a Chromatopac)NOTETo discontinue the performance inspection, press .1Connect t

7. Hardware Validation7-24RF-20A/20Axs6Press .The instrument is automatically given the following settings.After setting has finished, automatic zero

7.5 Detector Validation7-25RF-20A/20Axs710Press .Spectrum scanning for the emission wavelength takes place automatically.During spectrum scanning th

7. Hardware Validation7-26RF-20A/20Axs11Set a Chromatopac as follows.12Move the Chromatopac's pen position to the zero on the recording paper, t

7.5 Detector Validation7-27RF-20A/20Axs7Detection of the raman spectrum's peak and calculation of noise• The intensity value (F in the figure t

7. Hardware Validation7-28RF-20A/20Axs Check Procedure (When Using LCsolution)NOTETo discontinue the performance inspection, press .1Turn the power

7.5 Detector Validation7-29RF-20A/20Axs76Repeatedly press until [S/N CHECK] appears.7Press .The instrument is automatically given the following se

7. Hardware Validation7-30RF-20A/20Axs• When the raman spectrum peak is detected:Spectrum scanning ends normally. The instrument is automatically giv

7.5 Detector Validation7-31RF-20A/20Axs79Press .Measurement of noise starts automatically.During measurement, the screen to the right is displayed.A

Warning LabelsXXIRF-20A/20Axs Side of the InstrumentWarning label(Part No. 228-51557)

7. Hardware Validation7-32RF-20A/20AxsDetection of the raman spectrum's peak and calculation of noise• The intensity value (F in the figure to t

7.5 Detector Validation7-33RF-20A/20Axs77.5.7 Checking the Leak Sensor ObjectiveCheck the operation of the leak sensor.NOTETake care that the leak

7. Hardware Validation7-34RF-20A/20Axs4After about 10 seconds, press .When a leak is detected:When a leak is detected, [CHECK GOOD] is displayed.Whe

7.6 System Validation7-35RF-20A/20Axs77.6 System Validation• The LC system comprises many individual components. It is a complete system that allows

7. Hardware Validation7-36RF-20A/20Axs7.6.1 Validation of an Isocratic LC System ObjectiveAn analysis is performed on the LC system being validated

7.6 System Validation7-37RF-20A/20Axs7 Checking and Preparing the LC System1Check the connection status of each unit. Details on the connection of

7. Hardware Validation7-38RF-20A/20AxsFor a system that has been using a mixture of a water solution and an organic solvent as mobile phase, or water

7.6 System Validation7-39RF-20A/20Axs7Detector's display screen4Set the detector parameters.^ "Parameter Settings for Isocratic System Val

7. Hardware Validation7-40RF-20A/20Axs Parameter Settings for Isocratic System ValidationThe parameters to be set for the various components when va

7.6 System Validation7-41RF-20A/20Axs77.6.2 Validation of a Gradient LC System ObjectiveAn analysis is performed on the LC system being validated a

IntroductionXXIIRF-20A/20AxsAction for Environment (WEEE)To all users of Shimadzu equipment in the European Union:Equipment marked with this symbol in

7. Hardware Validation7-42RF-20A/20Axs Checking and Preparing the LC System1Check the connection status of each unit. Details on the connection of e

7.6 System Validation7-43RF-20A/20Axs7For a system that has been using a mixture of a water solution and an organic solvent as mobile phase, or wate

7. Hardware Validation7-44RF-20A/20AxsDetector's display screen4Set the detector parameters.^ "Parameter Settings for Isocratic System Vali

7.6 System Validation7-45RF-20A/20Axs7 Parameter Settings for Isocratic System ValidationThe parameters to be set for the various components when v

7. Hardware Validation7-46RF-20A/20Axs7.7 If Validation FailsShould the system fail to satisfy any of the system validation check criteria, or should

7.8 Reference Information7-47RF-20A/20Axs77.8 Reference InformationGenerally, the variations of this instrument conform to "7.5 Detector Valida

7. Hardware Validation7-48RF-20A/20Axs4. Calculation of the angle of turn required to move the emission grating to the 0 nm positionThe angle of turn

7.8 Reference Information7-49RF-20A/20Axs711. Movement of the excitation grating to the 507 nm positionScan the excitation side grating in the vicin

7. Hardware Validation7-50RF-20A/20Axs18. Calculation of the angle of turn required to move the emission grating to the 761 nm positionThe angle of t

7.8 Reference Information7-51RF-20A/20Axs724. Reading the 507 nm emission line spectrum at the emission sideScan the emission side grating in the vi

ContentsContents-1RF-20A/20AxsIntroduction ...

7. Hardware Validation7-52RF-20A/20Axs7.8.2 Automatic Checking Function of Wavelength Accuracy[EX CHECK] automatically checks the wavelength accuracy

88 MaintenanceContents8.1 Periodic Inspection and Maintenance ... 8-28.2 Inspection and Si

8. Maintenance8-2RF-20A/20Axs8.1 Periodic Inspection and MaintenanceIt is necessary to perform periodic inspections of this instrument to ensure its

8.1 Periodic Inspection and Maintenance8-3RF-20A/20Axs8NOTEThe guaranteed service life of the Xenon lamp is a cumulative operating time of 2000 hour

8. Maintenance8-4RF-20A/20AxsFig. 8.18.1.3 Check After Inspection and MaintenanceAfter inspection and maintenance, check any leakage during pumping.^

8.2 Inspection and Simple Washing of the Cell8-5RF-20A/20Axs88.2 Inspection and Simple Washing of the Cell8.2.1 Inspecting the Cell Nomenclature of

8. Maintenance8-6RF-20A/20Axs Inspecting the Cell1Turn the power to the instrument OFF and unplug the power plug.2Open the front cover and the key p

8.2 Inspection and Simple Washing of the Cell8-7RF-20A/20Axs8 Fitting the Flow Cell UnitFit the flow cell unit to the instrument by following the p

8. Maintenance8-8RF-20A/20Axs8.2.2 Simple Cleaning of the CellIf any air bubble or dirt is found on inspecting the cell, perform simple cleaning of t

8.2 Inspection and Simple Washing of the Cell8-9RF-20A/20Axs8 Simple Cleaning of the Cell1Fit the syringe adapter to the tip of the syringe.Fig. 8.

ContentsContents-2RF-20A/20AxsChapter 2 Parts Identification and Functions2.1 Front ...

8. Maintenance8-10RF-20A/20Axs4Fill the syringe with 2-propanol, then slowly push the syringe's plunger in.The methanol in the syringe flows int

8.3 Disassembling the Flow Cell Unit and Cleaning/Replacing Each Part8-11RF-20A/20Axs88.3 Disassembling the Flow Cell Unit and Cleaning/Replacing Ea

8. Maintenance8-12RF-20A/20AxsComponent parts of the flow cell unit (RF-20A)Fig. 8.13ScrewsCell coverPackingEmission mirrorSpacerO-ringEmission lensS

8.3 Disassembling the Flow Cell Unit and Cleaning/Replacing Each Part8-13RF-20A/20Axs8Component parts of the flow cell unit (RF-20AXS)Fig. 8.14Cell

8. Maintenance8-14RF-20A/20Axs8.3.1 Disassembling the Flow Cell UnitFig. 8.15 Disassembling the Flow Cell Unit1Turn the power to the instrument OFF

8.3 Disassembling the Flow Cell Unit and Cleaning/Replacing Each Part8-15RF-20A/20Axs87Remove the two screws on the top face of the flow cell unit,

8. Maintenance8-16RF-20A/20Axs• For the RF-20A:Using an implement with a fine point, such as a toothpick, remove the O-ring from the cell housing whi

8.3 Disassembling the Flow Cell Unit and Cleaning/Replacing Each Part8-17RF-20A/20Axs8RF-20AxsFig. 8.238.3.2 Cleaning the CellClean the cell removed

8. Maintenance8-18RF-20A/20Axs Fitting the CellNOTE• Fit the cell so that the inlet side and outlet side face toward the front face of the flow cell

8.3 Disassembling the Flow Cell Unit and Cleaning/Replacing Each Part8-19RF-20A/20Axs88.3.3 Cleaning the Emission LensParts used For the RF-20Axs:R

ContentsContents-3RF-20A/20Axs4.1.8 Setting Sensitivity ... 4-1

8. Maintenance8-20RF-20A/20Axs3Moisten a piece of clean gauze or wiper cloth with 2-propanol and use it to wipe off the dirt on the emission lens.∗ I

8.3 Disassembling the Flow Cell Unit and Cleaning/Replacing Each Part8-21RF-20A/20Axs88.3.4 Cleaning the Emission MirrorParts used For the RF-20Axs

8. Maintenance8-22RF-20A/20Axs For the RF-20A:1Unscrew the fixing screw for the emission mirror with a hexagon wrench (1.5 mm across flats). (The sc

8.3 Disassembling the Flow Cell Unit and Cleaning/Replacing Each Part8-23RF-20A/20Axs8 Fitting the Emission MirrorNOTE• Fit the emission mirror so

8. Maintenance8-24RF-20A/20Axs8.3.5 Cleaning the Excitation LensParts used Cleaning the Excitation Lens1Remove the fixing screw for the excitation l

8.3 Disassembling the Flow Cell Unit and Cleaning/Replacing Each Part8-25RF-20A/20Axs81Fit the excitation lens in the cell housing by following the

8. Maintenance8-26RF-20A/20Axs8.3.6 Cleaning the Excitation MirrorParts used Cleaning the Excitation Mirror1Remove the fixing screw for the excitati

8.3 Disassembling the Flow Cell Unit and Cleaning/Replacing Each Part8-27RF-20A/20Axs8 Fitting the Excitation MirrorNOTE• Fit the excitation mirror

8. Maintenance8-28RF-20A/20Axs2Fit the syringe adapter to the tip of the syringe.Fig. 8.383Fit the outlet tube to the outlet coupling 1.6-0.8C.Lead t

8.3 Disassembling the Flow Cell Unit and Cleaning/Replacing Each Part8-29RF-20A/20Axs88Fit the tubing from the column onto the inlet coupling 1.6-0.

ContentsContents-4RF-20A/20Axs Setting the Flow Cell Temperature [CELL TEMP] (RF-20Axs Only) ...5-17 Setting the Response [RESP

8. Maintenance8-30RF-20A/20Axs8.4 Inspecting/Replacing the Xenon Lamp!WARNING• When handling a Xenon lamp, always wear the following protective gear:

8.4 Inspecting/Replacing the Xenon Lamp8-31RF-20A/20Axs88.4.1 Replacing the Xenon LampParts used1Turn the power to the instrument OFF, unplug the po

8. Maintenance8-32RF-20A/20Axs5Unscrew the two screws on the front face of the lamp unit.∗ The two screws need not be removed.Fig. 8.446Hold the lamp

8.4 Inspecting/Replacing the Xenon Lamp8-33RF-20A/20Axs88Unscrew the knurled nut at the top of the Xenon lamp and remove the cord terminal from the

8. Maintenance8-34RF-20A/20Axs12Once the Xenon lamp has been fitted in the lamp unit, fit the parts that were removed by following the procedure for

8.5 Replacing the Fuse8-35RF-20A/20Axs88.5 Replacing the FuseBe sure to use fuses of the following type and capacity for replacement.Parts used8.5.1

8. Maintenance8-36RF-20A/20Axs3Fit a new fuse in the fuse holder and fit the fuse holder in the instrument.Fig. 8.534Using a screwdriver, turn the fu

8.6 Replacing the Filter8-37RF-20A/20Axs88.6 Replacing the FilterThis instrument has filters fitted inside the right side face and the front cover.

8. Maintenance8-38RF-20A/20Axs8.6.2 Replacing the Filter in the Front Cover1Turn the power to the instrument OFF and unplug the power plug. 2Open the

8.7 Performing Wavelength Calibration8-39RF-20A/20Axs88.7 Performing Wavelength CalibrationWavelength calibration and a wavelength accuracy check ar

ContentsContents-5RF-20A/20Axs Calibration Support Group ... 5-35

8. Maintenance8-40RF-20A/20Axs For the RF-20Axs:NOTEThe time required for wavelength calibration is approximately 30 minutes.To discontinue the wave

8.7 Performing Wavelength Calibration8-41RF-20A/20Axs86Enter the password.When the password matches, [WAVE CALIB] is displayed. ∗ If the password en

8. Maintenance8-42RF-20A/20AxsOn completion of wavelength calibration the wavelength accuracy check starts automatically and the screen to the right

8.7 Performing Wavelength Calibration8-43RF-20A/20Axs8When LCsolution is used:1Exit LCsolution.2Turn the power to the system controller OFF. 3Set [0

8. Maintenance8-44RF-20A/20Axs5Using the pump, pump water into the flow cell (flow rate: 1 mL/min).^ "8.2 Inspection and Simple Washing of the C

8.7 Performing Wavelength Calibration8-45RF-20A/20Axs812Enter the cell number set for the instrument with the numeric keys and press .Cell numbers

8. Maintenance8-46RF-20A/20Axs• If there is an abnormality:The screen to the right appears.^ "6.2 Dealing with Error Messages" P.6-513Press

8.7 Performing Wavelength Calibration8-47RF-20A/20Axs8When LCsolution is used:1Exit LCsolution.2Turn the power to the instrument and the system cont

8. Maintenance8-48RF-20A/20Axs8.8 Cleaning the ExteriorIf the main cover or front panel becomes dirty, wipe it clean with a soft dry cloth or tissue

99 Technical InformationContents9.1 Installation ...

IntroductionIRF-20A/20AxsIntroductionRead this manual thoroughly before using the instrument.Thank you for purchasing this instrument.This manual desc

ContentsContents-6RF-20A/20Axs5.4 Creating Time Programs ...5-545.4.1 Lis

9. Technical Information9-2RF-20A/20Axs9.1 Installation9.1.1 Installation Site Suitable Sites and PreparationTo ensure safe operation, install the i

9.1 Installation9-3RF-20A/20Axs9 Required Installation SpaceTypical system configurations and required installation spaces are shown in the figures

9. Technical Information9-4RF-20A/20Axs9.1.2 Installation Removing the Shipping ScrewsIn order to prevent shock during transportation, the instrumen

9.1 Installation9-5RF-20A/20Axs9 Stacking BracketsThe use of commercially available stacking brackets is recommended. These brackets limit the poss

9. Technical Information9-6RF-20A/20Axs9.1.3 Power Supply ConnectionThe following table shows the power supply voltage, power consumption, and freque

9.1 Installation9-7RF-20A/20Axs9 Connection to Power OutletFig. 9.61Insert the connector end of the power cord into the power cord connector at the

9. Technical Information9-8RF-20A/20Axs9.1.4 Prior to PlumbingMany different types of tubing and connector are used to plumb the instrument at instal

9.1 Installation9-9RF-20A/20Axs9Cutting SUS tubing:1Position the provided file (for cutting SUS tubing, Part No.: 670-18928-02) diagonally against t

9. Technical Information9-10RF-20A/20Axs Connecting Tubings1Mount a male nut and a ferrule to the tubing.Fig. 9.112Insert the end of the tubing, wit

9.1 Installation9-11RF-20A/20Axs9• For an SUS male nut:Use the open-end wrench (provided) to tighten and loosen the nut.If the nut is to be connecte

ContentsContents-7RF-20A/20Axs5.8.2 Wiring ...

9. Technical Information9-12RF-20A/20Axs Protective PlugsThe inlets and outlets of the units are fitted with protective plugs (bushings, stop plugs,

9.1 Installation9-13RF-20A/20Axs99.1.5 PlumbingThe necessary plumbing is as follows:Fig. 9.15!CAUTION• Before plumbing, turn OFF the power supply to

9. Technical Information9-14RF-20A/20Axs Waste Container PreparationBefore plumbing, prepare a waste container, or a waste container made of metal,

9.1 Installation9-15RF-20A/20Axs93Fit male nuts PEEK to both ends of the PEEK tubing.4Loosen and remove the stop plug that is fitted to the column o

9. Technical Information9-16RF-20A/20Axs Plumbing the Cell Outlet Tube1Fit a male nut PEEK onto one of the ends of the tubing for plumbing provided

9.1 Installation9-17RF-20A/20Axs9 Securing the Tubing for Plumbing1Fit the two tubings for plumbing into the grooves of the tubing clamp.Fig. 9.212

9. Technical Information9-18RF-20A/20AxsLowest unit:1Insert the drain OUT, STD into leakage drain outlet from the front of the instrument.2Turn the d

9.1 Installation9-19RF-20A/20Axs9Second unit from bottom:NOTELeaks from the column oven are drained separately (refer to the column oven instruction

9. Technical Information9-20RF-20A/20AxsInstallation on top of the column oven:NOTEWhen the lower unit has no leakage hole ("Fig. 9.28"), c

9.1 Installation9-21RF-20A/20Axs9 Front Cover Installation1After performing the plumbing, install the front cover by following the removal procedur

ContentsContents-8RF-20A/20Axs7.5.5 Checking Wavelength Accuracy ... 7-11 Objective

9. Technical Information9-22RF-20A/20Axs9.1.6 Installation of Manual Injector and ColumnUse the manual injectors listed below.Fit the manual injector

9.1 Installation9-23RF-20A/20Axs99.1.7 Flow Line PlumbingThe figure below shows and example of the basic system plumbing when this instrument is use

9. Technical Information9-24RF-20A/20Axs2Install a male nut (with long bushing) and ferrule on one end of each of the two waste liquid tubing section

9.1 Installation9-25RF-20A/20Axs9 Plumbing Between Pump Unit and Manual InjectorNOTEProvide the SUS tubing with some extra length.If there is no ex

9. Technical Information9-26RF-20A/20Axs Plumbing Between Manual Injector and ColumnNOTEProvide the SUS tubing with some extra length.If the left do

9.1 Installation9-27RF-20A/20Axs99.1.8 Wiring ConnectorsUse those connectors among the above that are needed for the system. Connection instruction

9. Technical Information9-28RF-20A/20Axs Connecting the Optical CableThe optical cable provided with this instrument is a two-way assembly for both

9.1 Installation9-29RF-20A/20Axs9Fig. 9.44!CAUTION• Do not bend the optical cable with a radius of less than 35 mm.• When inserting and removing the

9. Technical Information9-30RF-20A/20Axs Connecting to a System Controller1Referring to "Connecting the Optical Cable" P.9-28, connect the

9.1 Installation9-31RF-20A/20Axs9 Connecting to a ChromatopacFig. 9.461Connect the provided signal cable to analog output connector 1 or 2. Then co

ContentsContents-9RF-20A/20Axs Inspecting the Cell ...

9. Technical Information9-32RF-20A/20Axs Connecting to a RecorderFig. 9.471Connect the signal cable provided as an accessory to analog output connec

9.1 Installation9-33RF-20A/20Axs99.1.9 Fitting the Xenon Lamp1Turn the power to the instrument OFF and unplug the power plug. 2Open the front cover

9. Technical Information9-34RF-20A/20Axs3Unscrew the knurled screw (white) securing lamp cover 1, then remove lamp cover 1 and the filter.∗ The knurl

9.1 Installation9-35RF-20A/20Axs96Hold the lamp unit's handle and pull the unit out of the instrument as shown in the figure to the right.Fig.

9. Technical Information9-36RF-20A/20Axs9Fit the Xenon lamp in the lamp unit.∗ The Xenon lamp comes with a lamp holder.Fit the lamp by aligning the t

9.1 Installation9-37RF-20A/20Axs99.1.10 Fitting the Low-Pressure Hg (Mercury) Lamp (RF-20A Only)When performing wavelength calibration or checking t

9. Technical Information9-38RF-20A/20Axs4Fit the low-pressure Hg (mercury) lamp into the lamp unit so that the slit in the low-pressure Hg (mercury)

9.1 Installation9-39RF-20A/20Axs97Tighten the two screws on the front face of the lamp unit to secure the lamp unit to the instrument.Fig. 9.608Clos

9. Technical Information9-40RF-20A/20Axs9.2 Specifications∗1: Indicates the reproducibility performance when the power is turned ON in the single wav

9.2 Specifications9-41RF-20A/20Axs9Item RF-20A RF-20AxsZero Adjustment Auto zero function, baseline shift functionSpectrum Scanning FunctionOf the t

ContentsContents-10RF-20A/20AxsChapter 9 Technical Information9.1 Installation ...

9. Technical Information9-42RF-20A/20Axs9.3 Maintenance Parts9.3.1 Consumable Parts9.3.2 Replacement Parts Optical SystemPart Name Part No. RemarkXe

9.3 Maintenance Parts9-43RF-20A/20Axs9 Flow Cell/Plumbing PartsEM photo-sensor ASSY 228-51007-41 EM HP sensor with wire harnessHg mirror photo-sens

9. Technical Information9-44RF-20A/20Axs Electrical Parts OthersPart Name Part No. RemarkFuse 5AT, 250 V 072-02004-23 −Printed circuit board, RF20-

9.4 Introduction to the HPLC System9-45RF-20A/20Axs99.4 Introduction to the HPLC SystemThe Prominence LC (LC-20A) series components are for use with

9. Technical Information9-46RF-20A/20Axs9.4.2 Example of Autosampler System (1)Centralized control of all the components by a CBM-20Alite system cont

9.4 Introduction to the HPLC System9-47RF-20A/20Axs99.4.3 Example of Autosampler System (2)The CBM-20A system controller can control a maximum of ei

9. Technical Information9-48RF-20A/20Axs9.5 Mobile Phase Characteristics(1)Solvent(*)η ≤ 0.5 cp, B.P. > 45 °C(**)η ≤ 0.5 cp, B.P. < 45 °C(2)Sou

9.5 Mobile Phase Characteristics9-49RF-20A/20Axs935 n-Butanol LC 210 1.397 118 2.6 3.9 0.7 20.1 17.5 8.336 n-Propanol LC 240 1.385 97 1.9 4.0 0.82 m

9. Technical Information9-50RF-20A/20AxsSources: A.M.Krstulovic, P.R.Brown: Reversed-Phase High-Performance Liquid Chromatography, Wiley Interscience

IndexIndex-1RF-20A/20AxsIndexSymbolsλ MODE ... 5-13AAnalog output connector 1 ...

ContentsContents-11RF-20A/20Axs9.3.1 Consumable Parts ... 9-429.

IndexIndex-2RF-20A/20AxsGGAIN ... 4-17, 5-13, 5-54Guaranteed service life ...

IndexIndex-3RF-20A/20AxsSENS ... 4-18, 5-13, 5-54SENS COMP ... 5-

IndexIndex-4RF-20A/20AxsThis page is intentionally left blank.

ContentsContents-12RF-20A/20AxsThis page is intentionally left blank.

11 ConfigurationContents1.1 Overview ... 1-21.2

1. Configuration1-2RF-20A/20Axs1.1 OverviewThis instrument is a spectrofluorometric detector for high-performance liquid chromatograph developed for

1.2 Features1-3RF-20A/20Axs11.2 Features• Excellent Signal-to-Noise Ratio PerformanceExcellent S/N ratio performance has been achieved through impro

IntroductionIIRF-20A/20AxsWarranty and After-Sales ServiceWarranty 1. Period:Please consult your Shimadzu representative for information about the per

1. Configuration1-4RF-20A/20Axs1.3 Component PartsThis instrument consists of the parts listed below. Check the parts and their quantities after unpa

1.4 Optional Parts1-5RF-20A/20Axs11.4 Optional Parts Optional CellsChanging the standard cell to the following optional cells enables use as a dete

1. Configuration1-6RF-20A/20Axs Photomultiplier for RF-20A Photomultiplier for RF-20AxsPart Name Part No. RemarkPhotomultiplier R928-08 200-75021On

22 Parts Identification and FunctionsContents2.1 Front ...

2. Parts Identification and Functions2-2RF-20A/20Axs2.1 FrontFront coverOpen this cover to remove or install the flow cell or to do the tubing work.A

2.2 Behind Front Cover, Top Panel and Left Side2-3RF-20A/20Axs22.2 Behind Front Cover, Top Panel and Left SideCell inlet tube (with blue tubing)Conn

2. Parts Identification and Functions2-4RF-20A/20Axs2.3 Right Side and Base PanelShipping screw (painted red)To prevent shock during transportation.

2.4 Back2-5RF-20A/20Axs22.4 BackPower cord connectorFor connecting the power cord.Fuse holdersFuses are set in these.External input/output terminals

2. Parts Identification and Functions2-6RF-20A/20Axs2.5 Names and Functions of Displays and KeypadThis instrument is controlled with the keys on the

2.5 Names and Functions of Displays and Keypad2-7RF-20A/20Axs2No. Display or Indicator Function1Indicator functionShows the function of the Xenon la

Warranty and After-Sales ServiceIIIRF-20A/20Axs11) Consumable itemsNote: Recording media such as floppy disks and CD-ROMs are considered consumable it

2. Parts Identification and Functions2-8RF-20A/20Axs2.5.2 KeypadThis instrument is operated, and settings are made, by using the operation keys on it

2.5 Names and Functions of Displays and Keypad2-9RF-20A/20Axs2 List of Keys That Can Be Operated on Pressing the Display KeyKey Name FunctionEdit k

2. Parts Identification and Functions2-10RF-20A/20AxsThis page is intentionally left blank.

33 PreparationContents3.1 Cautions on Operation ... 3-23.2 Turning

3. Preparation3-2RF-20A/20Axs3.1 Cautions on Operation Cautions Prior to Operation• When high-sensitivity analysis is required, light the Xenon lamp

3.2 Turning the Power ON/OFF3-3RF-20A/20Axs33.2 Turning the Power ON/OFF1Press the power switch to turn the power ON.Press it again to turn the powe

3. Preparation3-4RF-20A/20Axs6A memory check is executed.7If there is no abnormality, the screen shown to the right is displayed.Initial screen in th

3.2 Turning the Power ON/OFF3-5RF-20A/20Axs3 Example Error Message DisplayIf an alarm sounds and [NOT PROTECTED] is displayed:This error message is

3. Preparation3-6RF-20A/20AxsThis page is intentionally left blank.

44 Basic OperationContents4.1 Measuring in the Single Wavelength Mode ... 4-24.2 Measuring in the D

IntroductionIVRF-20A/20AxsSafety Instructions• To ensure safe operation of the instrument, read these Safety Instructions carefully before use.• Obser

4. Basic Operation4-2RF-20A/20Axs4.1 Measuring in the Single Wavelength ModeThis section explains the procedure for measurement in the single wavelen

4.1 Measuring in the Single Wavelength Mode4-3RF-20A/20Axs44Press repeatedly until [λ MODE] is displayed.5Enter and press .This sets the measurem

4. Basic Operation4-4RF-20A/20Axs3Press .[ch1] will be displayed.In the initial status, the excitation wavelength can be entered.4Enter the excitatio

4.1 Measuring in the Single Wavelength Mode4-5RF-20A/20Axs44.1.3 Setting the Analog Output ConnectorsSet whether to connect the Chromatopac (integra

4. Basic Operation4-6RF-20A/20AxsAnalog output connector 1 (RF-20A)4Press repeatedly until [ANALOG1 MODE] or [ANALOG2 MODE] is displayed.∗ When set

4.1 Measuring in the Single Wavelength Mode4-7RF-20A/20Axs46Press twice.Analog output connector output mode setting ends and you are returned to th

4. Basic Operation4-8RF-20A/20Axs Details of Output Range SettingsThese details are common to analog output connectors 1 and 2.* When a baseline off

4.1 Measuring in the Single Wavelength Mode4-9RF-20A/20Axs42Press .[PARAMETER] will be displayed.3Press .[ch1] will be displayed.Analog output conne

4. Basic Operation4-10RF-20A/20Axs6Press twice.Setting of the output range for the analog output connector ends and you are returned to the initial

4.1 Measuring in the Single Wavelength Mode4-11RF-20A/20Axs44.1.5 Setting Baseline Offset ValuesSet baseline offset values for analog output connect

Safety InstructionsVRF-20A/20Axs Installation Site Precautions!WARNING• The solvents used in high performance liquid chromatograph are flammable and

4. Basic Operation4-12RF-20A/20Axs Setting the Baseline Offset Values of Analog Output Connectors1Press .The initial screen will be displayed.2Press

4.1 Measuring in the Single Wavelength Mode4-13RF-20A/20Axs45Enter the offset value (units: mV) from the numeric keys and press .This will change t

4. Basic Operation4-14RF-20A/20Axs4.1.6 Setting the Response (Response Speed)This instrument achieves an improved S/N ratio by using a digital filter

4.1 Measuring in the Single Wavelength Mode4-15RF-20A/20Axs43Press .[ch1] will be displayed.4Press repeatedly until [RESPONSE] is displayed.5Enter

4. Basic Operation4-16RF-20A/20Axs4.1.7 Setting GainThis is the procedure for setting gain. By combining the setting of sensitivity and gain, you can

4.1 Measuring in the Single Wavelength Mode4-17RF-20A/20Axs43Press .[ch1] will be displayed.4Press repeatedly until [GAIN] is displayed.5Enter the

4. Basic Operation4-18RF-20A/20AxsThe settings for sensitivity are shown below.On shipping from the factory, the setting is [2] (MED).1Press .The ini

4.1 Measuring in the Single Wavelength Mode4-19RF-20A/20Axs44.1.9 Setting the Flow Cell Temperature (RF-20Axs Only)Set the temperature of the flow c

4. Basic Operation4-20RF-20A/20Axs3Press .[ch1] will be displayed.4Press repeatedly until [CELL TEMP] is displayed.5Enter the set value from the nu

4.2 Measuring in the Dual Wavelength Mode4-21RF-20A/20Axs44.2 Measuring in the Dual Wavelength ModeThis section explains the procedure for measureme

IntroductionVIRF-20A/20Axs Installation PrecautionsTo ensure safe operation, contact your Shimadzu representative if product installation, adjustment

4. Basic Operation4-22RF-20A/20Axs4Press repeatedly until [λ MODE] is displayed.5Enter and press .This sets the measurement mode to dual wavelengt

4.2 Measuring in the Dual Wavelength Mode4-23RF-20A/20Axs42Press .[PARAMETER] will be displayed.3Press .[ch1] will be displayed.In the initial statu

4. Basic Operation4-24RF-20A/20Axs10Enter the emission wavelength with the numeric keys, then press .This sets the emission wavelength.11Press twic

4.2 Measuring in the Dual Wavelength Mode4-25RF-20A/20Axs44.2.3 Setting the Analog Output ConnectorsSet whether to connect the Chromatopac (integrat

4. Basic Operation4-26RF-20A/20Axs1Press .The initial screen will be displayed.2Press .[PARAMETER] will be displayed.3Press .[ch1] will be displayed.

4.2 Measuring in the Dual Wavelength Mode4-27RF-20A/20Axs4Analog output connector 1 (RF-20Axs)Analog output connector 2 (RF-20Axs)5Enter the set val

4. Basic Operation4-28RF-20A/20Axs4.2.4 Setting the Output RangesBefore setting the output ranges, set analog output connectors 1 and 2 in accordance

4.2 Measuring in the Dual Wavelength Mode4-29RF-20A/20Axs4 Details of Output Range SettingsThese details are common to analog output connectors 1 a

4. Basic Operation4-30RF-20A/20Axs2Press .[PARAMETER] will be displayed.3Press .[ch1] will be displayed.Analog output connector 14Press repeatedly

4.2 Measuring in the Dual Wavelength Mode4-31RF-20A/20Axs44.2.5 Setting Baseline Offset ValuesSet baseline offset values for analog output connector

Safety InstructionsVIIRF-20A/20Axs!WARNING• Do not place heavy objects on the power cord, and keep any hot items away.The cord could be damaged, resul

4. Basic Operation4-32RF-20A/20Axs Setting the Baseline Offset Values of Analog Output Connectors1Press .The initial screen will be displayed.2Press

4.2 Measuring in the Dual Wavelength Mode4-33RF-20A/20Axs46Press twice.Setting of baseline offset values for analog output connectors ends and you

4. Basic Operation4-34RF-20A/20Axs1Press .The initial screen will be displayed.2Press .[PARAMETER] will be displayed.3Press .[ch1] will be displayed.

4.2 Measuring in the Dual Wavelength Mode4-35RF-20A/20Axs4NOTEIf a slow response value (time constant) is set, responsiveness is adversely affected

4. Basic Operation4-36RF-20A/20Axs4.2.7 Setting GainThis is the procedure for setting gain. By combining the setting of sensitivity and gain, you can

4.2 Measuring in the Dual Wavelength Mode4-37RF-20A/20Axs43Press .[ch1] will be displayed.4Press repeatedly until [GAIN] is displayed.5Enter the s

4. Basic Operation4-38RF-20A/20Axs4.2.8 Setting SensitivityThis is the procedure for setting sensitivity. By combining the setting of sensitivity and

4.2 Measuring in the Dual Wavelength Mode4-39RF-20A/20Axs44Press repeatedly until [SENS] is displayed.5Enter the set value from the numeric keys an

4. Basic Operation4-40RF-20A/20Axs4.2.9 Setting the Flow Cell Temperature (RF-20Axs Only)Set the temperature of the flow cell.The fluorescent intensi

4.2 Measuring in the Dual Wavelength Mode4-41RF-20A/20Axs43Press .[ch1] will be displayed.4Press repeatedly until [CELL TEMP] is displayed.5Enter